Speedily get sections as slim or thick as your software demands, with no stressing about getting rid of or distorting beneficial tissue samples.
Megatome is usually a vibrating microtome created to area a wide choice of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With superior blade vibrating frequency and minimized blade deflection, Megatome enables higher-throughput tissue sectioning with uniform surface area profile, along with nominal tissue problems and knowledge decline.
Antibodies might get weeks to diffuse via only a few millimeters of tissue, having a steep labeling gradient from surface to core.
Jogging the CLI straight from a Gradle process is not currently supported. A distribution must be established by using gradlew :j2d-cli:distZip to produce a zip file that contains anything necessary to run.
SE makes use of a rotational electric field to disperse highly electromobile molecules (which include antibodies or surfactant micelles) in the course of a porous sample devoid of detrimental electrically charged structures within the tissue. This allows two-4 day clearing of intact organs,
Our preformulated EasyIndex Answer raises and homogenize the refractive index of delipidated tissue samples, rendering them fully clear. This permits mild penetration into the sample and makes sure the acquisition of substantial-resolution, in-target image details.
Megatome is usually a novel microtome that permits for high-precision sectioning of a wide array of tissue samples – from organoids, to arrays of animal organs, to intact human brain hemispheres – with negligible tissue injury and information reduction.
It’s the perfect time to improve your microtome to Megatome. With exact large-frequency slicing for an unmatched range of sample measurements and types – from organoids and tumors to expanded tissues, sample arrays, and intact primate organs – Megatome is optimized for various applications.
Fully delipidate complete mouse brains or comparably sized samples in only one day with SmartBatch+, or in a single week with our passive clearing package.
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SHIELD avoids the variability of hydrogel embedding and the information loss from PFA preservation, preserving specimens for several rounds of processing.
Leverage the Distinct+ tissue clearing approach, as well as eFLASH and patented stochastic electrotransport systems, to promptly apparent and label whole organs. Key highlights megatomi.com and attributes contain:
Our novel Defend tissue preservation strategy kinds intramolecular bonds employing polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.